Medical News

How the FluidFM ADD-ON brings much more "bio" in bioAFM

Would you wish to unlock distinctive organic apps on your AFM? Are you interested by single-cell experiments? So, FluidFM could possibly be the proper expertise that can assist you in your analysis.

Fluidic Drive Microscopy – Scientific Affect

Fluidic Drive Microscopy (FluidFM) expertise is a singular mixture of microfluidics and atomic power microscopy. Every patented (ie cantilever) FluidFM hole probe opens up many new functions for AFM customers. Since its invention in 2009, FluidFM has earned a fame for enabling unprecedented experiments in cell biology and biophysics.

The scientific attraction of FluidFM is already underlined by greater than 60 publications with a formidable common influence issue larger than eight.

With the rising variety of customers and methods at the moment put in in additional than 70 labs around the globe, we anticipate additional discoveries from the FluidFM neighborhood.

The partnerships we have now chosen with the three main producers of BioAFM, Bruker, JPK and Nanosurf, are one of many main causes for this speedy development within the variety of customers.

Picture 1: AFM companions of Cytosurge – carry FluidFM to the AFM neighborhood.

What precisely does FluidFM supply to an AFM consumer?

FluidFM – Go Past AFM Imaging

For customers of BioAFM, FluidFM opens many fascinating functions past imaging; starting from single-cell gene enhancing to a lot sooner mechanical measurements, equivalent to single-cell power spectroscopy (SCFS), as much as the direct printing of submicron function fashions.

Picture 2: FluidFM permits organic analysis with AFM – the injection of fabric into adherent cells.

Whereas it’s not unusual for brand spanking new customers of FluidFM to develop their distinctive utility, standard FluidFM functions embody:

Adhesion of particular person cells
Adhesion of easy micro organism
Colloidal spectroscopy
Single cell isolation (choose and place)

Determine three: DAPI printed on DNA coated glass

Current highlights of FluidFM

FluidFM permits my staff to successfully seize the adhesive forces of microbial pathogens and seek for new anti-adhesion molecules. I feel FluidFM has the potential to shed a whole lot of gentle on antimicrobial methods. "

Prof. Yves Dufrene, UC Leuven, Belgium

FluidFM allows distinctive and artistic AFM analysis, which frequently leads to high-impact publications; here’s a collection of three landmark publications of the previous couple of months:

The invention of a brand new mechanism of adhesion of the infamous pathogen Candida Albicans

Breakthrough methodology for detecting single additional or intracellular ions with a modified FluidFM configuration
M. Aramesh, C. Forró, L. Dorwling-Carter, I. Lüchtefeld, T. Schlotter, S. J. Ihle, I. Shorubalko, V. Hosseini, D. Momotenko, T. Zambelli, E. Klotzsch and J. Vörös. Localized detection of ions and biomolecules utilizing a managed – scanning nanopore scanning microscope. (2019) Nature Nanotechnology. doi: 10.1038 / s41565-019-0493-z

A scientific unicellular mechanical research of foliar microbiota

Nonetheless, how can FluidFM be added to your AFM system?

The FluidFM ADD-ON – tailored to your AFM

Determine 5: FluidFM probes – simple to make use of and tailored to every system. The FluidFM chip is pre-glued on a FluidFM adapter tailored to a selected AFM. The FluidFM adapter options an built-in fluid reservoir and pneumatic connections.

A FluidFM purposeful probe requires a liquid reservoir, pneumatic connections and correct micrometer mounting. Because of this, we have now developed FluidFM ADD-ON options in cooperation with AFM producers. This allowed us to fastidiously look at the geometric and bodily necessities of the FluidFM components in addition to the AFM system.

The result’s a singular FluidFM probe holder mannequin for some AFM and customized FluidFM probes, together with the state-of-the-art FluidFM microfluidic management system.

Determine four: FluidFM System ADD-ON

FluidFM ADD-ON options are already out there for the next AFM households:

Bruker BioScope Resolve
JPK NanoWizard, CellHesion and ForceRobot households
Nanosurf FlexAFM and CoreAFM Households

If you want to know extra about the right way to add FluidFM to your current AFM, we look ahead to listening to from you: